Optimal differentiation of cells cannot be obtained in the stagnant environment within a culture dish. This fact was the reason to integrate the tissue carriers in a per(i)fusion system. The carriers are placed into new containers, which allow permanent per(i)fusion of medium. The system is used with a peristaltic pump and a thermo plate and does not depend on a CO₂ - incubator. To maintain a constant pH HEPES or Buffer All (Sigma) are added to the media. Alternatively Leibovitz or other CO₂-independent media can be used. Cells or tissues can be cultured for months without subculturing in a quality not observed before.  See proceedings. 

What is necessary for a per(i)fusion culture experiment?

The starter set (13 mm - Nr. 1307) is the first step. It is ready for use and includes tissue carriers, a per(i)fusion container, tubes with fittings, media bottles and screw caps.

A peristaltic pump, for example Type IPC-N8 from ISMATEC.

A thermo plate, for example Type ME 12501 from MEDAX Nagel.

A complete set including IPC-N8 pump, thermo plate, cover lid and precision thermometer is available through our company (Nr. 0007).